| Graham Chapman | Fish #1 / Obstetrician / Harry Blackitt / Wymer / Hordern / General / Narrator #2 / Dr. Livingstone / Strange woman / Eric / Coles / Chairman / Guest #4 / Arthur Jarrett / Geoffrey / Tony Bennett |
| John Cleese | Fish #2 / Dr. Spenser / Humphrey Williams / Sturridge / Ainsworth / Waiter / Man / Maitre D / Grim Reaper |
| Terry Gilliam | Fish #4 / Walters / Zulu announcer / M'Lady Joeline / Mr. Brown / Howard Katzenberg / Window Washer |
| Eric Idle | Fish #3 / Singer / Mr. Moore / Mrs. Blackitt / Watson / Atkison / Perkins / Victim #3 / Front End / Mrs. Hendy / Man in pink / Noel Coward / Gaston / Angela |
| Terry Jones | Fish #6 / Mum / Priest / Biggs / Sergeant / Strange man / Mrs. Brown / Mr. Creosote / Maria / Bert / Leaf #1 / Fiona Portland-Smyth |
| Michael Palin | Fish #5 / Mr. Pycroft / Dad / Narrator #1 / Chaplain / Carter / Spadger / Sergeant Major / Pakenham-Walsh / Rear End / Lady presenter / Mr. Marvin Hendy / Harry / Padre / Debbie Katzenberg / Window Wa |
| Carol Cleveland | Wife of Guest #4/Heaven Greeter |
| Judy Loe | Nurse #1 |
| Simon Jones | Cedric |
| Andrew MacLachlan | Groom |
| Valerie Whittington | Mrs. Moore |
| Mark Holmes | Severed head |
| Patricia Quinn | Mrs. Williams |